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neoBolt™ Bst DNA Polymerase

neoBolt™ Bst DNA Polymerase

Varizymes' neoBolt Bst DNA Polymerase (patent pending) is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), thermostable, Bst-like DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. NeoBolt Bst DNA Polymerase has increased sensitivity and speed relative to other Bst DNA polymerases, and can incorporate dUTP.

Ordering Information

ItemProduct NumberConcentration (U/mL)Amount (U)Price
neoBolt Bst DNA Polymerase9100S8,0005,000$170
neoBolt Bst DNA Polymerase9100L8,00010,000$330
neoBolt Bst DNA Polymerase (glycerol-free)9101InquireInquireInquire
  • Fast
  • Target Agnostic
  • Thermostable

Product Details

Please be sure to use the buffer provided with this product to ensure optimal results.

NeoBolt Bst DNA Polymerase is eligible for our OEM Customization Program. Contact us for more information.

This product is intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.

Properties

  • Only Bst DNA Polymerase on the market with robust reverse transcriptase activity and DNA polymerase activity.
  • No additional reverse transcriptase needed for amplification of RNA targets.
  • Thermostable, working temperature range 64-72 °C.
  • Tolerant to inhibitors.
  • Use neoBolt Bst DNA Polymerase to develop LAMP assays with high sensitivity and specificity.
  • Buffer composition: 50% glycerol, 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.05% Tween-20, 0.05% NP-40 substitute, pH=7.5.
  • Storage temperature: -20 °C.

Certificates of Analysis & SDS

Resources

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The Varizymes Advantages

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Fast

mLAMP results in about 15 minutes.

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Target Agnostic

Amplify DNA or RNA targets with no added reverse transcriptase required.

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Thermostable

Performs at up to 72 °C, enabling better performance with structured RNAs.