We got outstanding sequencing results when using Varizymes SwifTag™ Tn5 2.0 as the first step of our NGS library prep.
Compared to samples prepared with an enzymatic fragmentation method, followed by end-repair and adapter ligation (prepped at the same time and included in the same sequencing run), direct incorporation of the adapter using SwifTag Tn5 produced higher mapping efficiency, sensitivity, and reproducibility.
Jesse Riordan, University of Iowa