MOLECULAR DIAGNOSTICS
Fast. Simple. Accurate.

Rapid, easy-to-use, and portable diagnostic assays are critical to making point-of-care and field-based testing more accessible.

Choose From Two Platforms:

ChromaLAMP

✔ Single target per test
✔ Colorimetric detection
✔ Quick and easy protocol
✔ Economical

TORCH mLAMP

✔ Up to 4 targets per test
✔ Fluorescent detection
✔ Quick and easy protocol
✔ Economical

Introducing ChromaLAMP™
Single-Target Testing Made Easy.

Fast. Simple. Economical.

Varizymes' ChromaLAMP (Loop-mediated isothermal AMPlification) kits are a simple, fast, and economical way to test for a single pathogen species.

ChromaLAMP kits are powered by Varizymes' neoBolt™ Bst DNA Polymerase, an enzyme uniquely engineered for high strand-displacement and reverse transcriptase activity.

The test is quick and simple: lyse the sample and add to our proprietary master mix. Incubate at 68 °C for 30 minutes and look for the color change.

TORCH™ mLAMP
Multiple Pathogens. One Test.

Fast. Simple. Multiplexed.

Multiplex-Loop-mediated Isothermal AMPlification: A Better Way to Test.

TORCH multiplex LAMP (TORCH mLAMP) is a new way to rapidly and accurately test for several pathogens simultaneously, with minimal cost, equipment, and expertise.

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What is TORCH™ mLAMP?
Multiplex-Loop-mediated Isothermal AMPlification: A Better Way to Test.

TORCH™ mLAMP (TORCH multiplex Loop-mediated isothermal AMPlification) is a new way to rapidly and accurately test for several pathogens simultaneously, with minimal cost, equipment, and expertise.  Like our ChromaLAMP single-plex platform, TORCH mLAMP is powered by our proprietary neoBolt Bst DNA Polymerase, a strand-displacing polymerase with very high reverse transcriptase activity.

Since TORCH mLAMP uses multiple fluorescent dyes, it can detect more than one target at a time. In fact, TORCH supports the simultaneous detection of up to four targets, often including an internal control and (up to) 3 pathogens.

Common LAMP detection methods like turbidity, dyes, and gel electrophoresis are sequence-independent, limiting multi-target detection in a single tube. Lateral flow strips and melt analysis can detect multiple targets but require extra steps or specialized
equipment.

At Varizymes, we have developed an innovative approach, TORCH mLAMP (patent pending), to detect multiple targets in a single LAMP reaction. Now, get all the benefits of LAMP with the ability to test for up to 4 targets per sample. 

Benefits of using TORCH mLAMP

TORCH Multiplex LAMP (loop-mediated isothermal amplification, or mLAMP) is a simple, fast and economical way to test for several pathogens simultaneously.

For field-based testing, ease of use and portability are key. Combine unpurified sample with our pre-made, lyophilized master mix and test. A portable, battery-operated fluorometer will provide unambiguous results in a few minutes, onsite. There is no need to send samples to a reference lab.

Samples can often be tested as-is, without the need for time-consuming nucleic acid extraction. Our tests are as specific and sensitive as qPCR, but far faster and simpler to run.

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TORCH™ mLAMP is Ideal for:

  • Remote Field Testing
  • Wildlife Monitoring
  • Clinical & At-Home Diagnostics
  • Environmental Monitoring
  • Disease Outbreak Response

Transforming Human and Animal Diagnostics

Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets

Advantages of TORCH™ mLAMP Technology

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Uses standard LAMP primer design.
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Unlike other mLAMP methods, TORCH™does not require additional primers, probes, or extra steps such as melt analysis.
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Real-time detection of amplification products.
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Detection of up to 4 targets in a single mLAMP reaction (very similar to qPCR).
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Similar speed, sensitivity, and specificity to a traditional LAMP assay.
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Works for both DNA and RNA targets.
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Multiplexing can result in significant cost savings and improved testing efficiency.
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How Does TORCH mLAMP Work?

Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets

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This is a TORCH mLAMP workflow for a quadruplex assay. Sample is collected and placed in a tube containing a lyophilized master mix. In most cases, no nucleic acid extraction is necessary.

Reaction mix is incubated at a constant temperature — a thermal cycler is not required. Factorial amplification occurs via 6 primers that bind uniquely to 6-8 distinct regions of the target (colored bars).

Multiple rounds of strand displacement followed by primer annealing and polymerization form dumbell-like loop structures. These loop structures facilitate subsequent rounds of amplification producing very long (>20 kb) DNA concatamers with numerous repeats of the target sequence.

Amplification is factorial, producing detectable levels of amplified targets within 15-25 minutes. This occurs simultaneously for up to 4 targets in the same reaction. The entire process, from sample collection to results, takes less than 30 minutes and is all done on-site, at the point-of-care.