MOLECULAR DIAGNOSTICS
Fast. Simple. Accurate.
Rapid, easy-to-use and portable diagnostic assays are critical to making point-of-care and field-based testing more accessible.
What is TORCH™ mLAMP?
Multiplex-Loop-mediated Isothermal AMPlification: A Better Way to Test.
TORCH™ mLAMP (TORCH multiplex Loop-mediated isothermal AMPlification) is a new way to rapidly and accurately test for several pathogens simultaneously, with minimal cost, equipment and expertise. Like our ChromaLAMP single-plex platform, TORCH mLAMP is powered by our proprietary neoBolt Bst DNA Polymerase, a strand-displacing polymerase with very high reverse transcriptase activity.
Since TORCH mLAMP uses multiple fluorescent dyes, it can detect more than one target at a time. In fact, TORCH supports the simultaneous detection of up to four targets, often including an internal control and (up to) 3 pathogens.
Common LAMP detection methods like turbidity, dyes, and gel electrophoresis are sequence-independent, limiting multi-target detection in a single tube. Lateral flow strips and melt analysis can detect multiple targets but require extra steps or specialized
equipment.
At Varizymes, we have developed an innovative approach, TORCH mLAMP, to detect multiple targets in a single LAMP reaction. Now, get all the benefits of LAMP with the ability to test for up to 4 targets per sample.
Transforming Human and Animal Diagnostics
Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets
Advantages of TORCH™ mLAMP Technology
Nucleic acid extraction usually not required.
Uses standard LAMP primer design.
Unlike other mLAMP methods, TORCH does not require additional primers, probes, or extra steps such as melt analysis.
Real-time detection of amplification products.
Detection of up to 4 targets in a single mLAMP reaction (very similar to qPCR).
Similar speed, sensitivity, and specificity to a traditional LAMP assay.
Works for both DNA and RNA targets.
Multiplexing can result in significant cost savings and improved testing efficiency.
How Does TORCH mLAMP Work?
Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets
This is a TORCH mLAMP workflow for a quadruplex assay. Sample is collected and placed in a tube containing a lyophilized master mix. In most cases, no nucleic acid extraction is necessary.
Reaction mix is incubated at a constant temperature — a thermal cycler is not required. Factorial amplification occurs via 6 primers (colored bars) that bind uniquely to 6-8 distinct regions of the target.
Multiple rounds of strand displacement followed by primer annealing and polymerization form dumbell-like loop structures. These loop structures facilitate subsequent rounds of amplification producing very long (>20 kb) DNA concatamers with numerous repeats of the target sequence.
Amplification is factorial, producing detectable levels of amplified targets within 15-25 minutes. This occurs simultaneously for up to 4 targets in the same reaction. The entire process, from sample collection to results, takes less than 30 minutes and is all done on-site, at the point-of-care.
