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Cod Uracil-DNA Glycosylase

$280.00

Is amplification carry-over contamination ruining your assay sensitivity?

Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carry over contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. cUNG is the only commercially available UNG that is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carry-over contamination and the generation of false positives and inaccurate quantification.

cUNG is also eligible for our OEM Customization Program. Contact us for more info!

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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Optimal temperature: 37 °C

Heat inactivation: 55 °C for 5 minutes

Enables contamination control in PCR and other amplification methods

Does not degrade product after inactivation, enabling downstream use of the amplicon

Buffer composition: 50% glycerol, 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 1 mM EDTA, 0.1% Tween-20, pH 7.5 (alternate buffers available as custom orders)

Storage temperature: -20 °C

$280.00
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