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Cod Uracil-DNA Glycosylase

Cod Uracil-DNA Glycosylase

Varizymes' Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carryover contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. Varizymes' cUNG is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carryover contamination and the generation of false positives and inaccurate quantification.

Ordering Information

ItemProduct NumberConcentration (U/mL)Amount (U)Price
Cod Uracil-DNA Glycosylase4000S50,0002,000$280
Cod Uracil-DNA Glycosylase4000L50,00010,000$1,120
  • Robust
  • Safe
  • Effective

Product Details

Cod Uracil-DNA Glycosylase is eligible for our OEM Customization Program. Contact us for more information.

This product is intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.

Properties

  • Optimal temperature: 37 °C.
  • Heat inactivation: 55 °C for 5 minutes.
  • Enables contamination control in PCR, LAMP and other amplification methods.
  • Does not degrade product after inactivation, enabling downstream use of the amplicon.
  • Buffer composition: 50% glycerol, 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Tween-20, pH 7.5 (alternate buffers available as custom orders).
  • Storage temperature: -20 °C.

Certificates of Analysis & SDS

Resources

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The Varizymes Advantages

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Robust

Complete digestion of uracil-containing amplicons.

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Safe

Fully inactivated at 55 °C for 5 minutes.

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Effective

Does not degrade product after inactivation, enabling downstream use of the amplicon.