The RNA-guided endonuclease Cas9, associated with Type II CRISPR/Cas systems, site-specifically digests DNA using a single guide RNA (sgRNA) which it binds to direct it to the complementary sequence. Varizymes offers two traditional versions of Streptococcus pyogenes Cas9 nuclease: CRISPR/Cas9, ideal for in vitro DNA digestion, and CRISPR/Cas9 NLS, for in vivo nuclear localization. Made-to-order CRISPR/Cas9  is also available (contact for details), and a guide for sgRNA design is available from Addgene.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.